Article In: orcid, cienciavitae

Nanoaggregate-forming lipid-conjugated AS1411 aptamer AS a promising tumor-targeted delivery system of anticancer agents in vitro

Nanomedicine: Nanotechnology, Biology and Medicine

Carvalho, Josué; Lopes-Nunes, Jéssica; et al. — 2021 — Elsevier

Key information

Authors:

Carvalho, Josué; Lopes-Nunes, Jéssica; Vialet, Brune; Rosado, Tiago; Gallardo, Eugenia; Vale, João; Eloy, Catarina; Campello, Paula (Maria Paula Cordeiro Crespo Cabral Campello Aboim de Barros); Paulo, António (António Manuel Rocha Paulo); et al.

Published in

August 2021

Abstract

Nanoparticles offer targeted delivery of drugs with minimal toxicity to surrounding healthy tissue and have great potential in the management of human papillomavirus (HPV)-related diseases. We synthesized lipid-modified AS1411 aptamers capable of forming nanoaggregates in solution containing Mg2+. The nanoaggregates presented suitable properties for pharmaceutical applications such as small size (100 nm), negative charge, and drug release. The nanoaggregates were loaded with acridine orange derivative C8 for its specific delivery into cervical cancer cell lines and HPV-positive tissue biopsies. This improved inhibition of HeLa proliferation and cell uptake without significantly affecting healthy cells. Finally, the nanoaggregates were incorporated in a gel formulation with promising tissue retention properties aiming at developing a local delivery strategy of the nanoaggregates in the female genital tract. Collectively, these findings suggest that the nanoformulation protocol has great potential for the delivery of both anticancer and antiviral agents, becoming a novel modality for cervical cancer management.

Publication details

Authors in the community:

Publication version

AO - Author's Original

Publisher

Elsevier

Link to the publisher's version

https://www.sciencedirect.com/journal/nanomedicine-nanotechnology-biology-and-medicine

Title of the publication container

Nanomedicine: Nanotechnology, Biology and Medicine

First page or article number

102429

Volume

36

ISSN

1549-9634

DOI

10.1016/j.nano.2021.102429

WoS (Web of Science)

WOS:000687935400013

Fields of Science and Technology (FOS)

chemical-sciences - Chemical sciences

Keywords

  • Nanoaggregates
  • Drug delivery
  • AS1411 G-quadruplex
  • Cervical cancer
  • HPV

Publication language (ISO code)

eng - English

Alternative identifier (URI)

https://doi.org/10.1016/j.nano.2021.102429

Rights type:

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