Artigo De: orcid, cienciavitae, scopus

Interrogating the Role of Receptor-Mediated Mechanisms: Biological Fate of Peptide-Functionalized Radiolabeled Gold Nanoparticles in Tumor Mice

Bioconjugate Chemistry

Silva, F.; Zambre, A.; Paulo, Antonio — 2016 — ACS Publications

Informações chave

Autores:

Silva, F.; Zambre, A.; Campello, M.P.C. (Maria Paula Cordeiro Crespo Cabral Campello Aboim de Barros); Gano, L. (Maria de Lurdes Barrela Patrício Gano); Santos, I.; Ferraria, A.M. (Ana Maria da Conceição Ferraria); Ferreira, M.J. (Maria João Gomes Ferreira); et al; Paulo, Antonio (António Manuel Rocha Paulo)

Publicado em

20/04/2016

Resumo

To get a better insight on the transport mechanism of peptide-conjugated nanoparticles to tumors, we performed in vivo biological studies of bombesin (BBN) peptide functionalized gold nanoparticles (AuNPs) in human prostate tumor bearing mice. Initially, we sought to compare AuNPs with thiol derivatives of acyclic and macrocyclic chelators of DTPA and DOTA types. The DTPA derivatives were unable to provide a stable coordination of 67Ga, and therefore, the functionalization with the BBN analogues was pursued for the DOTA-containing AuNPs. The DOTA-coated AuNPs were functionalized with BBN[7–14] using a unidentate cysteine group or a bidentate thioctic group to attach the peptide. AuNPs functionalized with thioctic-BBN displayed the highest in vitro cellular internalization (≈ 25%, 15 min) in gastrin releasing peptide (GRP) receptor expressing cancer cells. However, these results fail to translate to in vivo tumor uptake. Biodistribution studies following intravenous (IV) and intraperitoneal (IP) administration of nanoconjugates in tumor bearing mice indicated that the presence of BBN influences to some degree the biological profile of the nanoconstructs. For IV administration, the receptor-mediated pathway appears to be outweighed by the EPR effect. By contrast, in IP administration, it is reasoned that the GRPr-mediated mechanism plays a role in pancreas uptake.

Detalhes da publicação

Autores da comunidade :

Versão da publicação

AO - Versão original do autor

Editora

ACS Publications

Ligação para a versão da editora

https://pubs.acs.org/bc

Título do contentor da publicação

Bioconjugate Chemistry

Primeira página ou número de artigo

1153

Última página

1164

Volume

27

Fascículo

4

DOI

10.1021/acs.bioconjchem.6b00102

WoS (Web of Science)

WOS:000374812600033

Domínio Científico (FOS)

chemical-sciences - Química

Idioma da publicação (código ISO)

eng - Inglês

Identificador alternativo (URI)

http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000374812600033&KeyUID=WOS:000374812600033

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