Artigo De: orcid, cienciavitae, scopus
Interrogating the Role of Receptor-Mediated Mechanisms: Biological Fate of Peptide-Functionalized Radiolabeled Gold Nanoparticles in Tumor Mice
Bioconjugate Chemistry
2016 — ACS Publications
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Autores:
Publicado em
20/04/2016
Resumo
To get a better insight on the transport mechanism of peptide-conjugated nanoparticles to tumors, we performed in vivo biological studies of bombesin (BBN) peptide functionalized gold nanoparticles (AuNPs) in human prostate tumor bearing mice. Initially, we sought to compare AuNPs with thiol derivatives of acyclic and macrocyclic chelators of DTPA and DOTA types. The DTPA derivatives were unable to provide a stable coordination of 67Ga, and therefore, the functionalization with the BBN analogues was pursued for the DOTA-containing AuNPs. The DOTA-coated AuNPs were functionalized with BBN[7–14] using a unidentate cysteine group or a bidentate thioctic group to attach the peptide. AuNPs functionalized with thioctic-BBN displayed the highest in vitro cellular internalization (≈ 25%, 15 min) in gastrin releasing peptide (GRP) receptor expressing cancer cells. However, these results fail to translate to in vivo tumor uptake. Biodistribution studies following intravenous (IV) and intraperitoneal (IP) administration of nanoconjugates in tumor bearing mice indicated that the presence of BBN influences to some degree the biological profile of the nanoconstructs. For IV administration, the receptor-mediated pathway appears to be outweighed by the EPR effect. By contrast, in IP administration, it is reasoned that the GRPr-mediated mechanism plays a role in pancreas uptake.
Detalhes da publicação
Autores da comunidade :
Ana Maria da Conceição Ferraria
ist31350
Maria João Gomes Ferreira
ist31162
António Manuel Rocha Paulo
ist126677
Versão da publicação
AO - Versão original do autor
Editora
ACS Publications
Ligação para a versão da editora
https://pubs.acs.org/bc
Título do contentor da publicação
Bioconjugate Chemistry
Primeira página ou número de artigo
1153
Última página
1164
Volume
27
Fascículo
4
WoS (Web of Science)
Domínio Científico (FOS)
chemical-sciences - Química
Idioma da publicação (código ISO)
eng - Inglês
Identificador alternativo (URI)
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000374812600033&KeyUID=WOS:000374812600033
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