Artigo De: orcid, cienciavitae, scopus

In vitro/in vivo “peeling” of multilayered aminocarboxylate gold nanoparticles evidenced by a kinetically stable 99mTc-label

Dalton Transactions

Silva, Francisco; Gano, Lurdes; Kannan, Raghuraman — 2017 — Royal Society of Chemistry

Informações chave

Autores:

Silva, Francisco; Gano, Lurdes (Maria de Lurdes Barrela Patrício Gano); Cabral Campello, Maria Paula (Maria Paula Cordeiro Crespo Cabral Campello Aboim de Barros); Marques, Rosa (Rosa Maria Salgueiro Marques); Prudencio, Isabel (Maria Isabel Garrido Prudêncio); Zambre, Ajit; Upendran, Anandhi; Paulo, Antonio (António Manuel Rocha Paulo); Kannan, Raghuraman

Publicado em

01/01/2017

Resumo

A thiolated bombesin peptide was conjugated to Au-DTDTPA nanoconstructs to obtain BBN-Au-DTDTPA targeted to the gastrin releasing peptide receptor (GRPr). Different analytical techniques showed that this conjugate shares similar physico-chemical properties with Au-DTDTPA; HPLC and XPS analyses corroborated the attachment of the bioactive peptide to the AuNPs surface. Competitive binding assays in PC3 cancer cells showed that these BBN-containing AuNPs have high affinity for GRPr. BBN-Au-DTDTPA was successfully radiolabeled with 99mTc and showed high in vitro stability towards different biological media and substrates, except for glutathione (GSH). In vitro and in vivo studies, based on gamma-counting (99mTc content) and neutron activation analysis (Au content), indicated the release of the DTDTPA coating from the AuNPs. Probably, the “peeling” of the layered-aminocarboxylate coating is GSH-mediated and involves the cleavage of the DTDTPA disulfide bonds and/or Au–S bonds. These results render BBN-Au-DTDTPA an interesting platform deserving further evaluation in target-specific GSH-mediated drug delivery.

Detalhes da publicação

Autores da comunidade :

Versão da publicação

AO - Versão original do autor

Editora

Royal Society of Chemistry

Ligação para a versão da editora

https://pubs.rsc.org/en/journals/journal/dt

Título do contentor da publicação

Dalton Transactions

Primeira página ou número de artigo

14583

Última página

14583

Volume

46

Fascículo

42

DOI

10.1039/c7dt00864c

WoS (Web of Science)

WOS:000414243600014

Domínio Científico (FOS)

chemical-sciences - Química

Idioma da publicação (código ISO)

eng - Inglês

Identificador alternativo (URI)

http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:28485418&KeyUID=MEDLINE:28485418

Acesso à publicação:

Acesso apenas a metadados