Article In: orcid, cienciavitae, scopus

In vitro/in vivo “peeling” of multilayered aminocarboxylate gold nanoparticles evidenced by a kinetically stable 99mTc-label

Dalton Transactions

Silva, Francisco; Gano, Lurdes; Kannan, Raghuraman — 2017 — Royal Society of Chemistry

Key information

Authors:

Silva, Francisco; Gano, Lurdes (Maria de Lurdes Barrela Patrício Gano); Cabral Campello, Maria Paula (Maria Paula Cordeiro Crespo Cabral Campello Aboim de Barros); Marques, Rosa (Rosa Maria Salgueiro Marques); Prudencio, Isabel (Maria Isabel Garrido Prudêncio); Zambre, Ajit; Upendran, Anandhi; Paulo, Antonio (António Manuel Rocha Paulo); Kannan, Raghuraman

Published in

01/01/2017

Abstract

A thiolated bombesin peptide was conjugated to Au-DTDTPA nanoconstructs to obtain BBN-Au-DTDTPA targeted to the gastrin releasing peptide receptor (GRPr). Different analytical techniques showed that this conjugate shares similar physico-chemical properties with Au-DTDTPA; HPLC and XPS analyses corroborated the attachment of the bioactive peptide to the AuNPs surface. Competitive binding assays in PC3 cancer cells showed that these BBN-containing AuNPs have high affinity for GRPr. BBN-Au-DTDTPA was successfully radiolabeled with 99mTc and showed high in vitro stability towards different biological media and substrates, except for glutathione (GSH). In vitro and in vivo studies, based on gamma-counting (99mTc content) and neutron activation analysis (Au content), indicated the release of the DTDTPA coating from the AuNPs. Probably, the “peeling” of the layered-aminocarboxylate coating is GSH-mediated and involves the cleavage of the DTDTPA disulfide bonds and/or Au–S bonds. These results render BBN-Au-DTDTPA an interesting platform deserving further evaluation in target-specific GSH-mediated drug delivery.

Publication details

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Publication version

AO - Author's Original

Publisher

Royal Society of Chemistry

Link to the publisher's version

https://pubs.rsc.org/en/journals/journal/dt

Title of the publication container

Dalton Transactions

First page or article number

14583

Last page

14583

Volume

46

Issue

42

DOI

10.1039/c7dt00864c

WoS (Web of Science)

WOS:000414243600014

Fields of Science and Technology (FOS)

chemical-sciences - Chemical sciences

Publication language (ISO code)

eng - English

Alternative identifier (URI)

http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:28485418&KeyUID=MEDLINE:28485418

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