Article In: orcid, cienciavitae

Radiolabeled Mannosylated Dextran Derivatives Bearing an NIR-Fluorophore for Sentinel Lymph Node Imaging

Bioconjugate Chemistry

Morais, M.; Campello, M.P.C.; Santos, I. — 2014 — ACS Publications

Key information

Authors:

Morais, M.; Campello, M.P.C. (Maria Paula Cordeiro Crespo Cabral Campello Aboim de Barros); Xavier, C.; Heemskerk, J.; Correia, J.D.G. (João Domingos Galamba Correia); Lahoutte, T.; Caveliers, V.; Hernot, S.; Santos, I. (Isabel da Graça Rego dos Santos)

Published in

2014

Abstract

Current methods for sentinel lymph node (SLN) mapping involve the use of radioactivity detection with technetium-99m sulfur colloid and/or visually guided identification using a blue dye. To overcome the kinetic variations of two individual imaging agents through the lymphatic system, we report herein on two multifunctional macromolecules, 5a and 6a, that contain a radionuclide (99mTc or 68Ga) and a near-infrared (NIR) reporter for pre- and/or intraoperative SLN mapping by nuclear and NIR optical imaging techniques. Both bimodal probes are dextran-based polymers (10 kDa) functionalized with pyrazole-diamine (Pz) or 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) chelating units for labeling with fac-[99mTc(CO)3]+ or 68Ga(III), respectively, mannose units for receptor targeting, and NIR fluorophore units for optical imaging. The probes allowed a clear visualization of the popliteal node by single-photon emission computed tomography (SPECT/CT) or positron emission tomography (PET/CT), as well as real-time optically guided excision. Biodistribution studies confirmed that both macromolecules present a significant accumulation in the popliteal node (5a: 3.87 ± 0.63% IA/organ; 6a: 1.04 ± 0.26% IA/organ), with minimal spread to other organs. The multifunctional nanoplatforms display a popliteal extraction efficiency >90%, highlighting their potential to be further explored as dual imaging agents.

Publication details

Authors in the community:

Publication version

AO - Author's Original

Publisher

ACS Publications

Link to the publisher's version

https://pubs.acs.org/toc/bcches/25/11

Title of the publication container

Bioconjugate Chemistry

First page or article number

1963

Last page

1970

Volume

25

Issue

11

DOI

10.1021/bc500336a

WoS (Web of Science)

WOS:000345309000007

Fields of Science and Technology (FOS)

chemical-sciences - Chemical sciences

Publication language (ISO code)

eng - English

Alternative identifier (URI)

http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000345309000007&KeyUID=WOS:000345309000007

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