Assessment of β-carotene content, cell physiology and morphology of the yellow yeast $\textit{Rhodotorula glutinis}$ mutant 400A15 using flow cytometry

01/08/2013

<jats:title>Abstract</jats:title> <jats:p>Flow cytometry was used to assess β-carotene content, cell membrane permeability, cell size and granularity in Rhodotorula glutinis mutant 400A15 grown under different oxygen transfer coefficients (k  L  a) and carbon to nitrogen ratios (C/N). A Doehlert distribution was used in order to select the best conditions that induced the highest carotenoids production. The highest β-carotene content (0.79 mg g−1 DCW) at the lowest k  L  a and C/N (5 × 10−3 s−1 and 11.3 respectively). Under these conditions, the biomass concentration attained 18.60 g L−1. The highest ratio of cells with permeabilised membranes (2.6 %), and the highest cell size and granularity were also obtained under these conditions. It was observed that C/N showed a stronger influence than the k  L  a on the measured cell parameters.</jats:p>

Autores da comunidade :

• 

  M

  Maria Beatriz Pinto Pereira Palma Nobre

  ist136051

  Afiliações e fontes

  De: cienciavitae

  ID: 1237430

VoR - Versão publicada

Oxford University Press (OUP)

Journal of Industrial Microbiology and Biotechnology

865

875

40

8

1476-5535

10.1007/s10295-013-1278-2

chemical-sciences - Química

Palavras-chave

• Applied Microbiology and Biotechnology
• Biotechnology
• Bioengineering

eng - Inglês

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