Article In: cienciavitae
Assessment of β-carotene content, cell physiology and morphology of the yellow yeast $\textit{Rhodotorula glutinis}$ mutant 400A15 using flow cytometry
Journal of Industrial Microbiology and Biotechnology
— 2013 — Oxford University Press (OUP)
Key information
Authors:
Published in
08/01/2013
Abstract
<jats:title>Abstract</jats:title> <jats:p>Flow cytometry was used to assess β-carotene content, cell membrane permeability, cell size and granularity in Rhodotorula glutinis mutant 400A15 grown under different oxygen transfer coefficients (k L a) and carbon to nitrogen ratios (C/N). A Doehlert distribution was used in order to select the best conditions that induced the highest carotenoids production. The highest β-carotene content (0.79 mg g−1 DCW) at the lowest k L a and C/N (5 × 10−3 s−1 and 11.3 respectively). Under these conditions, the biomass concentration attained 18.60 g L−1. The highest ratio of cells with permeabilised membranes (2.6 %), and the highest cell size and granularity were also obtained under these conditions. It was observed that C/N showed a stronger influence than the k L a on the measured cell parameters.</jats:p>
Publication details
Authors in the community:
Publication version
VoR - Version of Record
Publisher
Oxford University Press (OUP)
Title of the publication container
Journal of Industrial Microbiology and Biotechnology
First page or article number
865
Last page
875
Volume
40
Issue
8
ISSN
1476-5535
Fields of Science and Technology (FOS)
chemical-sciences - Chemical sciences
Keywords
- Applied Microbiology and Biotechnology
- Biotechnology
- Bioengineering
Publication language (ISO code)
eng - English
Alternative identifier (URI)
http://www.scopus.com/inward/record.url?eid=2-s2.0-84880775863&partnerID=MN8TOARS
Rights type:
Only metadata available