Bovine serum albumin adsorption onto 316L stainless steel and alumina: a comparative study using depletion, protein radiolabeling, quartz crystal microbalance and atomic force microscopy

31/10/2008

The importance of protein adsorption on biomaterials is widely recognized, but the dependence of the adsorption results on the chosen technique has not been much addressed. The objective of this work is to compare adsorption data obtained using several techniques under experimental conditions as closely as possible. Two case studies were investigated: adsorption of bovine serum albumin (BSA) onto 316L stainless steel (SS) and onto alumina. Both materials were used as powders and plates, whose characterization was done through zeta potential (ZP) measurements. The experimental techniques were depletion, protein radiolabeling, quartz crystal microbalance with dissipation (QCM-D) and atomic force microscopy (AFM). The adsorption isotherms obtained with depletion and QCM-D techniques, although quantitatively different, present some similarities in shape. Both techniques suggest the existence of a compact end-on monolayer of protein on the SS surface, while on the alumina surface a less dense side-on monolayer is formed at lower BSA concentration, followed by a second layer at higher concentration. AFM topographical characterization of the protein films adsorbed on both materials confirms those findings. Further use of AFM in determining the thickness of the film adsorbed on SS yielded values in good agreement with the QDM-D results. Different surface charges measured on powders and plates do not seem to affect adsorption. Protein radiolabeling seems to be the least reliable technique because it yields, for both materials, adsorption values higher than those from the other techniques. In the case of SS, the difference amounts to one order of magnitude. Copyright © 2008 John Wiley & Sons, Ltd.

Autores da comunidade :

• 

  M

  Montsant Pellice Gispert

  ist46582

• 

  B

  Benilde de Jesus Vieira Saramago

  ist11322

  Afiliações e fontes

  IST > CQE

  Centro de Química Estrutural

  IST > DEQ

  Departamento de Engenharia Química

  De: orcid

  ID: 10661764

• 

  A

  Ana Paula Valagão Amadeu do Serro

  ist134419

  Afiliações e fontes

  IST > CQE

  Centro de Química Estrutural

  IST > DEQ

  Departamento de Engenharia Química

• 

  R

  Rogério Anacleto Cordeiro Colaço

  ist13267

  Afiliações e fontes

  De: scopus

  ID: 2-s2.0-56749168579

VoR - Versão publicada

Wiley

https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/sia.2929

Surface and Interface Analysis

1529

1537

40

12

10.1002/sia.2929

materials-engineering - Engenharia dos Materiais

Palavras-chave

• BSA adsorption
• stainless steel
• alumina
• radiolabeling
• quartz crystal microbalance
• depletion technique
• atomic force microscopy

eng - Inglês