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Bovine serum albumin adsorption onto 316L stainless steel and alumina: a comparative study using depletion, protein radiolabeling, quartz crystal microbalance and atomic force microscopy

Surface and Interface Analysis

Gispert, M.P.; Saramago, B.; Rogério Anacleto Cordeiro — 2008 — Wiley

Key information

Authors:

Gispert, M.P. (Montsant Pellice Gispert); Saramago, B. (Benilde de Jesus Vieira Saramago); Serro, A.P. (Ana Paula Valagão Amadeu do Serro); Colačo, R.; Rogério Anacleto Cordeiro (Rogério Anacleto Cordeiro Colaço)

Published in

10/31/2008

Abstract

The importance of protein adsorption on biomaterials is widely recognized, but the dependence of the adsorption results on the chosen technique has not been much addressed. The objective of this work is to compare adsorption data obtained using several techniques under experimental conditions as closely as possible. Two case studies were investigated: adsorption of bovine serum albumin (BSA) onto 316L stainless steel (SS) and onto alumina. Both materials were used as powders and plates, whose characterization was done through zeta potential (ZP) measurements. The experimental techniques were depletion, protein radiolabeling, quartz crystal microbalance with dissipation (QCM-D) and atomic force microscopy (AFM). The adsorption isotherms obtained with depletion and QCM-D techniques, although quantitatively different, present some similarities in shape. Both techniques suggest the existence of a compact end-on monolayer of protein on the SS surface, while on the alumina surface a less dense side-on monolayer is formed at lower BSA concentration, followed by a second layer at higher concentration. AFM topographical characterization of the protein films adsorbed on both materials confirms those findings. Further use of AFM in determining the thickness of the film adsorbed on SS yielded values in good agreement with the QDM-D results. Different surface charges measured on powders and plates do not seem to affect adsorption. Protein radiolabeling seems to be the least reliable technique because it yields, for both materials, adsorption values higher than those from the other techniques. In the case of SS, the difference amounts to one order of magnitude. Copyright © 2008 John Wiley & Sons, Ltd.

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Publication version

VoR - Version of Record

Publisher

Wiley

Link to the publisher's version

https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/sia.2929

Title of the publication container

Surface and Interface Analysis

First page or article number

1529

Last page

1537

Volume

40

Issue

12

DOI

10.1002/sia.2929

Fields of Science and Technology (FOS)

materials-engineering - Materials engineering

Keywords

  • BSA adsorption
  • stainless steel
  • alumina
  • radiolabeling
  • quartz crystal microbalance
  • depletion technique
  • atomic force microscopy

Publication language (ISO code)

eng - English

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