Conference In: cienciavitae
Cell culture bioreactor manufacturing, from material selection to numerical models
Materiais 2022
— 2022 — MDPI
Key information
Authors:
Published in
04/11/2022
Abstract
Cell culture bioreactors play a paramount role in obtaining successful cell cultures that mature into tissue-like structures. Cellular growth is accomplished by supplying the seeded cells with sufficient nutrients while performing an adequate removal of cellular metabolic waste products [1]. At the same time, it is necessary for the cellular environment to maintain adequate biological levels of pH, temperature, gas concentration (O2, CO2), and hydrostatic pressure. The current work presents a new methodology to create a shareable and easily reproducible cell culture bioreactor based on open source technologies, accessible materials, and ubiquitous construction techniques. Our bioreactor designs are continuously supported by corresponding digital twin numerical models to optimize their operation in current tissue engineering processes [2]. Numerical models also play a key role in understanding the local conditions imposed and consequent cellular effects, especially when cellular environmental conditions become unfavorable or are very difficult to measure and control. Specifically, our research is focused on modeling the delivery of external stimulation modalities, such as electromagnetic, fluid flow, wall shear stress, and hydrostatic pressure. Our overall goal is for our predictions to ultimately help to obtain critical insights into the environmental cues that lead cells to differentiate, proliferate, grow, and, finally, mature into tissues of interest. As part of this project, we will present the developed bioreactor designs that will allow the validation of the digital twin models proposed. We will also present a detailed analysis of new developments and opportunities for numerical models and bioreactor in tissue engineering applications.
Publication details
Authors in the community:
João Carlos Fernandes da Silva
ist165105
Publication version
AO - Author's Original
Publisher
MDPI
Link to the publisher's version
https://www.mdpi.com/2673-4605/8/1/50
Title of the publication container
Materiais 2022
Location of the conference
Portugal
Conference date start
04/11/2022
Fields of Science and Technology (FOS)
health-biotechnology - Medical biotechnology
Keywords
- 3D printing
- biocompatible materials
- perfusion bioreactor
- numerical models
- digital twins
Publication language (ISO code)
eng - English
Rights type:
Open access