Yellow coloured mats from lava tubes of La Palma (Canary Islands, Spain) are dominated by metabolically active Actinobacteria

31/01/2018

<jats:title>Abstract</jats:title><jats:p>Microbial diversity in lava tubes from Canary Islands (Spain) has never been explored thus far offering a unique opportunity to study subsurface microbiology. Abundant yellow coloured mats developing on coralloid speleothems in a lava tube from La Palma Islands were studied by next-generation sequencing and DNA/RNA clone library analyses for investigating both total and metabolically active bacteria. In addition, morphological and mineralogical characterization was performed by field emission scanning electron microscopy (FESEM), micro-computed tomography, X-ray diffraction and infrared spectroscopy to contextualize sequence data. This approach showed that the coralloid speleothems consist of banded siliceous stalactites composed of opal-A and hydrated halloysite. Analytical pyrolysis was also conducted to infer the possible origin of cave wall pigmentation, revealing that lignin degradation compounds can contribute to speleothem colour. Our RNA-based study showed for the first time that members of the phylum <jats:italic>Actinobacteria</jats:italic>, with 55% of the clones belonging to <jats:italic>Euzebyales</jats:italic> order, were metabolically active components of yellow mats. In contrast, the DNA clone library revealed that around 45% of clones were affiliated to <jats:italic>Proteobacteria</jats:italic>. Composition of microbial phyla obtained by NGS reinforced the DNA clone library data at the phylum level, in which <jats:italic>Proteobacteria</jats:italic> was the most abundant phylum followed by <jats:italic>Actinobacteria</jats:italic>.</jats:p>

Autores da comunidade :

• 

  M

  Manuel Francisco Costa Pereira

  ist13225

  Afiliações e fontes

  IST > CERENA

  Centro de Recursos Naturais e Ambiente

  IST > CEPGIST

  Centro de Petrologia e Geoquímica

  IST > DECivil

  Departamento de Engenharia Civil, Arquitetura e Ambiente

  De: cienciavitae

  ID: 451673

VoR - Versão publicada

Springer Science and Business Media LLC

Scientific Reports

1944

8

1

2045-2322

10.1038/S41598-018-20393-2

earth-and-related-environmental-sciences - Ciências da Terra e Ciências do Ambiente

Palavras-chave

• Multidisciplinary

eng - Inglês

https://publons.com/publon/1171068/