Síntese de ómega-3 e ácidos gordos para a produção de biodiesel pela microalga marinha Crypthecodinium cohnii e estudo de métodos sustentáveis para a sua extração

July 5, 2022

In this study, the microalgae \textit{Crypthecodinium cohnii} was cultivated in order to produce high concentrations of docosahexaenoic acid and fatty acids for biodiesel production. In the assay I the fatty acid concentration obtained was very low due to oxygen limiting conditions. In trial II, the hydrolysis of molasses was not complete, resulting in a poor medium in carbon source and not allowing the growth of biomass. In test III a biomass concentration of 18.65 g/L was obtained and a DHA content of 7.52 \% (m/m). To study the ASE method, an experimental planning was performed, varying the factors temperature and extraction time. Using hexane as solvent, the condition that obtained the highest yields was 40 °C and 11 minutes. The yields in lipids, TFA, ADH and FA for biodiesel production were 26.35, 21.37, 8.18 and 13.05 g/100 g of dry and ash free biomass, respectively. When using ethanol, the highest yields were obtained at 140 °C and 11 minutes - 18.96, 7.28 and 11.56 g/100 g of dry and ash free biomass in TFA, DHA and FA for biodiesel production, respectively. For the study of the SCE method, 5 assays with different pressure and temperature conditions were performed. The highest yield in lipids was obtained at 300 bar and 41.3 °C - 22,40 g/100g biomass. The highest yields in TFA, DHA and FA for biodiesel production - 18.39, 7.08 and 11.20 g/100g biomass, respectively - were obtained at 300 bar and 60 °C.

Authors in the community:

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  C

  Clara Picão Fernandes Marques Henriques

  ist187379

Supervisors of this institution:

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  M

  Maria Teresa Saraiva Lopes da Silva

  ist125740

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  M

  Maria Beatriz Pinto Pereira Palma Nobre

  ist136051

chemical-engineering - Chemical engineering

por - Portuguese

Instituto Superior Técnico